igf 1r rabbit anti rat polyclonal antibody  (Boster Bio)

Journal: Hypertension Research

Article Title: IGF-1 receptor cleavage in hypertension

doi: 10.1038/s41440-018-0023-7

Figure Lengend Snippet: Micrographs of aorta sections of WKY a , c and SHRs b , d after IHC labeling with primary antibodies against IGF-1R alpha a , b and beta c , d . Arrowheads show the endothelium, and arrows indicate the smooth muscle layer. Negative controls for IGF-1 alpha and beta IHC labeling are shown as smaller images. Means ± SD of the percentage of stained area for IGF-1R alpha e and beta f in tunica intima (endothelium) and tunica media (smooth muscle cell) layers after IHC. The scale bar is 500 μm. * p < 0.05 compared with WKY ( n = 4)

Article Snippet: Sections were blocked in 0.1% BSA and incubated with a mouse anti-rat IGF-1R alpha monoclonal antibody (1:400; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; catalog number sc-463) or rabbit anti-rat IGF-1R beta polyclonal antibody (1:100; Cell Signaling Technology Inc., Beverly, MA, USA, catalog number 3027) overnight at 4 °C.

Techniques: Labeling, Staining

Journal: Hypertension Research

Article Title: IGF-1 receptor cleavage in hypertension

doi: 10.1038/s41440-018-0023-7

Figure Lengend Snippet: Immunolabeling of WKY aorta sections with a primary antibody against the IGF-1R alpha subunit. Aortic rings were incubated with plasma from WKY a , b or SHRs c , d in the presence b , d or absence a , c of protease inhibitors (EDTA 10 mM, doxycycline; 11,3 μM) for 24 h. Representative images are shown a - d . Arrowheads indicate the endothelium, and arrows indicate the smooth muscle layer. The scale bar is 500 μm. Bar graphs show the means ± SD of the percentage of stained area using the same incubation conditions a - d in tunica intima (endothelium) e and tunica media (smooth muscle cell) layers f . Two to four different aorta sections per animal were labeled to determine the average per rat ( n = 4). The scale bar is 500 μm. * p < 0.05 compared with WKY plasma-incubated rings; # p < 0.05 and ## p < 0.01 compared with SHR plasma-incubated rings

Article Snippet: Sections were blocked in 0.1% BSA and incubated with a mouse anti-rat IGF-1R alpha monoclonal antibody (1:400; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; catalog number sc-463) or rabbit anti-rat IGF-1R beta polyclonal antibody (1:100; Cell Signaling Technology Inc., Beverly, MA, USA, catalog number 3027) overnight at 4 °C.

Techniques: Immunolabeling, Incubation, Clinical Proteomics, Staining, Labeling

Journal: Hypertension Research

Article Title: IGF-1 receptor cleavage in hypertension

doi: 10.1038/s41440-018-0023-7

Figure Lengend Snippet: Immunostaining of the aorta sections of WKY with a primary antibody against IGF-1R beta subunit. Aortic rings were incubated with plasma from WKY a , b or SHRs c , d in the presence b, d or absence a, c of protease inhibitors (EDTA 10 mM, doxycycline; 11.3 μM) for 24 h. Representative images are shown a - d . Arrowheads show the endothelium, and arrows show the smooth muscle layer. The scale bar is 500 μm. Bar graphs show the means ± SD of the percentage of stained area under the same incubation conditions a - d in tunica intima (endothelium) e and tunica media (smooth muscle cell) layers f . Two to four different aorta sections per animal were labeled to determine the average per rat ( n = 4). The scale bar is 500 μm

Article Snippet: Sections were blocked in 0.1% BSA and incubated with a mouse anti-rat IGF-1R alpha monoclonal antibody (1:400; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; catalog number sc-463) or rabbit anti-rat IGF-1R beta polyclonal antibody (1:100; Cell Signaling Technology Inc., Beverly, MA, USA, catalog number 3027) overnight at 4 °C.

Techniques: Immunostaining, Incubation, Clinical Proteomics, Staining, Labeling

Journal: Hypertension Research

Article Title: IGF-1 receptor cleavage in hypertension

doi: 10.1038/s41440-018-0023-7

Figure Lengend Snippet: p-AKT and peNOS levels in WKY and SHR aorta rings incubated in HEPES buffer at 37 °C for 20 min in the presence (IGF-1) or absence (NS) of 100 nM IGF-1. Representative images are shown a, c . An antibody against p-AKT recognized a band in the range of 60 kDa a , an antibody against peNOS recognized a band in the range of 140 kDa c , and the anti-beta-actin antibody recognized a band at 45 kDa a , c . The p-AKT and peNOS band densities were normalized to the respective beta-actin signal. Bar graphs show the means ± SD of the band densities for p-AKT b and peNOS d ( n = 4)

Article Snippet: Sections were blocked in 0.1% BSA and incubated with a mouse anti-rat IGF-1R alpha monoclonal antibody (1:400; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; catalog number sc-463) or rabbit anti-rat IGF-1R beta polyclonal antibody (1:100; Cell Signaling Technology Inc., Beverly, MA, USA, catalog number 3027) overnight at 4 °C.

Techniques: Incubation

Journal: Hypertension Research

Article Title: IGF-1 receptor cleavage in hypertension

doi: 10.1038/s41440-018-0023-7

Figure Lengend Snippet: Schematic diagram of the loss of IGF-1 receptor intracellular signaling via the proteolytic cleavage of extracellular receptor domains

Article Snippet: Sections were blocked in 0.1% BSA and incubated with a mouse anti-rat IGF-1R alpha monoclonal antibody (1:400; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA; catalog number sc-463) or rabbit anti-rat IGF-1R beta polyclonal antibody (1:100; Cell Signaling Technology Inc., Beverly, MA, USA, catalog number 3027) overnight at 4 °C.

Techniques: